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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Alpha‐actinin‐4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy

Fig. 4

Effects of ACTN4 on HTR8/SVneo cell invasion and migration. a TRITC-Phalloidin staining on si-NC and si-ACTN4 of HTR8/SVneo cells. Scale bar, 200 μm. b Representative images and quantification of cells from the HTR8/SVneo cells with invasion in the presence or absence of a Matrigel-coated membrane for 24 h. Scale bar: 200 μm. c HTR8/SVneo cells attached to the culture plates after wounding and treatment with ACTN4 siRNA. The percentage of wound closure was calculated at 24 h. Scale bar: 400 μm. All data are presented as the means ± SEM of three independent experiments. **P < 0.01

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