Fig. 1

ACTN4 expression in placental CTBs. a IHC staining of ACTN4 in the human first trimester placenta villi. CTBs were stained for CK7; Scale bars: 100 μm. b EdU labeling in explant cultures. Villous explants isolated from the first trimester placentas were treated with the control siRNA (si-NC) or ACTN4 siRNA (si-ACTN4). Representative pictures showed EdU (pink) labeling in the villous cytotrophoblasts of floating villi following 72-h treatment. Nuclei were counterstained with DAPI (blue). Scale bars: 100 μm. All data are presented as the means ± SEM of three independent experiments. ^*p < 0.05