Fig. 2
From: Hsp90 modulates human sperm capacitation via the Erk1/2 and p38 MAPK signaling pathways
Interaction between Hsp90 and its co-chaperone Cdc37 in human sperm. a. Hsp90 was detected in sperm lysates using western blotting and Co-IP with non-immune mouse IgG or antibodies against Cdc37. No interaction was observed between non-immune mouse IgG and Hsp90, whereas a clear interaction was evident between Hsp90 and Cdc37. 17-AAG disturbed the formation of the Hsp90-Cdc37 complex. Results are representative of three independent experiments using samples from three different individuals. b. The relative association of Hsp90 and Cdc37 with or without 5 µM 17-AAG during human sperm capacitation