Fig. 1
From: Hsp90 modulates human sperm capacitation via the Erk1/2 and p38 MAPK signaling pathways
17-AAG inhibits human sperm acrosome reaction and hyperactivation. a. The human sperm acrosome reaction was evaluated using PSA-FITC staining, a common method for assessing sperm capacitation based on alterations in sperm head staining. Acrosome reaction (AR) indicates capacitated and acrosome-reacted sperm, while acrosome integrity (AI) indicates uncapacitated sperm. b. The acrosome reaction percentage was significantly lower when human sperm were treated with 5 µM 17-AAG than vehicle control (*P < 0.05). c. Sperm motility and hyperactivation were analyzed using CASA. The percentage of sperm with hyperactivated motility was significantly lower when treated with 5 µM 17-AAG than vehicle control (*P < 0.05)