Table 1 Primers used in this study
Gene | primer | Sequence |
|---|---|---|
MEG3 | Forward | 5′- GGCAGGATCTGGCATAGAGG − 3’ |
Reverse | 5′- CGAGTCAGGAAGCAGTGGGTT-3’ | |
PTEN | Forward | 5′- ACCCACACGACGGGAAGACA − 3’ |
Reverse | 5′- CTGTTTGTGGAAGAACTCTACTTTGATATCAC − 3’ | |
β-actin | Forward | 5′- CATTCCAAATATGAGATGCGTTGT − 3’ |
Reverse | 5′- TGTGGACTTGGGAGAGGACT − 3’ | |
miR-93-5p | Forward | 5′- GTCACAAAGUGCUGUUCGUGC-3’ |
Reverse Transcription | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCTG − 3’ | |
U6 | Forward | 5′- CTCGCTTCGGCAGCACA-3’ |
Reverse | 5′- AACGCTTCACGAATTTGCGT-3’ |