Fig. 6

Over-expression of PTEN attenuated the role of miR-93-5p mimics in Leydig cells treated with LPS. (A) qPCR and western blot analysis of PTEN expression in Leydig cells treated with different concentrations of LPS (0, 50, 100, 200, 400 μg/ml) for 48 h. (B) qPCR and western blot analysis of PTEN expression in Leydig cells transfected with plvx-PTEN and plvx-NC for 48 h. (C) ELISA analysis of secretion of TNFα and IL6 in Leydig cells. (D) ELISA analysis of testosterone production in Leydig cells. (E) CCK8 analysis of cell viability in Leydig cells. (F) EdU analysis of cell proliferation in Leydig cells. For C-F, Leydig cells were divided into four groups: mimics NC, LPS + mimics NC, LPS+ miR-93-5p mimics and LPS + miR-93-5p mimics+plvx-PTEN. β-Actin was measured as an internal control for qPCR analysis. “**” represents p < 0.01, which indicates statistically significant differences between two groups in Student’s t-test