Fig. 4

Inhibition of miR-93-5p alleviated the role of silenced MEG3 in Leydig cells treated with LPS. (A) ELISA analysis of secretion of TNFα and IL6 in Leydig cells. (B) ELISA analysis of testosterone production in Leydig cells. (C) CCK8 analysis of cell viability in Leydig cells. (D) EdU analysis of cell proliferation in Leydig cells. For A-D, Leydig cells were divided into six groups: shNC+inhibitor NC, shMEG3 + inhibitor NC, shMEG3+ miR-93-5p inhibitor, shNC+inhibitor NC + LPS, shMEG3 + inhibitor NC + LPS, and shMEG3+ miR-93-5p inhibitor+LPS. “**” represents p < 0.01, which indicates statistically significant differences between two groups in Student’s t-test