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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Silencing of MEG3 attenuated the role of lipopolysaccharides by modulating the miR-93-5p/PTEN pathway in Leydig cells

Fig. 2

Silencing of MEG3 attenuated the role of LPS in Leydig cells. (A) qPCR analysis of MEG3 expression in Leydig cells treated with different concentrations of LPS (0, 50, 100, 200, 400 μg/ml) for 48 h. (B) qPCR analysis of MEG3 expression in Leydig cells transfected with siMEG3s and siNC for 48 h. (C) ELISA analysis of secretion of TNFα and IL6 in Leydig cells. (D) ELISA analysis of testosterone production in Leydig cells. (E) CCK8 analysis of cell viability in Leydig cells. (F) EdU analysis of cell proliferation in Leydig cells. For C-F, Leydig cells were divided into four groups: shNC, shMEG3, shNC+LPS and shMEG3 + LPS. β-Actin was measured as an internal control for qPCR analysis. “**” represents p < 0.01, which indicates statistically significant differences between two groups in Student’s t-test

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