Fig. 1

LPS induced dysfunction and inhibited cell growth in Leydig cells. (A) ELISA analysis of secretion of TNFα and IL6 in Leydig cells. (B) ELISA analysis of testosterone production in Leydig cells. (C) CCK8 analysis of cell viability in Leydig cells. (D) EdU analysis of cell proliferation in Leydig cells. (E) Western blot analysis of cell apoptosis in Leydig cells. For A-C, Leydig cells were treated with different concentrations of LPS (0, 50, 100, 200, 400 μg/ml) for 48 h. For D and E, Leydig cells were treated with LPS at a dose of 200 μg/ml for 48 h. β-Actin was measured as a loading control for western blot analysis. “**” represents p < 0.01, which indicates significantly statistic differences between two groups in Student’s t-test