Fig. 6

Effects of intra-ARC and intra-AVPV LV-TTF1-shRNA administration on female rat puberty. Twenty-one-day-old female rats were infected with LV-TTF1-shRNA or LV-NC in the ARC or AVPV nucleus. a-c) Anogenital distance, ovarian organ coefficient and uterine organ coefficient in the rats injected with the LV-TTF1-shRNA or NC in the ARC were measured at different stages. d-f Anogenital distance, ovarian organ coefficient and uterine organ coefficient in the rats transfected with the NC or LV-TTF1-shRNA in the AVPV were measured at different stages. g and i) The body weights of rats receiving virus transfection were measured beginning at PND25. h and j Cumulative percentages of vaginal openings (VOs) were determined every morning after the intra-AVPV LV-TTF1-shRNA and intra-ARC LV-TTF1-shRNA transfection. Ovarian organ coefficient = ovary weight (g)/body weight (g). Uterine organ coefficient = uterine weight (g)/body weight (g). The data are presented as the mean ± S.E.M. *P < 0.05