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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Downregulation of TTF1 in the rat hypothalamic ARC or AVPV nucleus inhibits Kiss1 and GnRH expression, leading to puberty delay

Fig. 3

TTF1 gene overexpression in GT1–7 cells promoted the expression of Kiss1. GT1–7 cells were infected with TTF1 overexpression plasmids for 72 h. a EGFP (green) was measured with immunofluorescence microscopy. Optical microscopy was used to obtain bright field images. b TTF1, Kiss1 and GnRH mRNA levels were analysed with qRT-PCR after transfection. Cells transfected with LV-EGFP were used as a negative control. c TTF1 and Kiss1 protein concentrations were determined by western blotting analysis. TTF1-OE, TTF1-overexpression group. NC, lentivirus negative control group. Scale bars, 200 μm a. The results are shown as the mean ± S.E.M. ***P < 0.001

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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