Fig. 2
From: Novel variants in DNAH9 lead to nonsyndromic severe asthenozoospermia
a The expression of DNAH9-mRNA in spermatozoon is significantly lower in F1 II-1 compared with normal control. b Immunofluorescence staining in spermatozoon from the DNAH9-mutated F1 II-1 and normal control. DNAH9 immunostaining (red) was primarily concentrated at the sperm neck and flagellum in a punctate pattern, while the immunostaining was decreased in the sperm of F1 II-1. Hoechst (blue) was stained as a nuclear marker. The anti-tubulin (green) was stained as a marker of flagellum