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Table 1 The relevant parameters of qRT-PCR

From: Irisin deletion induces a decrease in growth and fertility in mice

Gene Primers Accession number Tm(°C) qPCR efficiency(%)
GAPDH F:5′-GGAGCGAGATCCCTCCAAAAT-3′ Nm_002046.7 60 95.2
R:5′- GGCTGTTGTCATACTTCTCATGG-3′
Fndc5 F:5′ -TGGTAATCCCTGGACTGCAG-3′ NM_153756.3 60 92.7
R:5′- GGGTACAAGGAGATGGAGGG-3′
Akr1c18 F:5′- ACCAAATTGGCAATTGAAGCT-3′ NM_134066.3 60 89.6
R:5′- TGGGATCACTTCCTCACCTG-3′
Mamld1 F: 5′-TTCTGCAGCAGATGATGCAG-3’ NM_005491.5 60 90.3
R:5′- GAGGATCTTGCCTGCTAGTG-3’
Cyp19a1 F:5′- ATGTGGACGTGTTGACCCTTCT-3’ NM_000103.4 60 91.5
R:5′- AGGAGAGCT TGCCATGCATCAA-3’
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