Table 1 The relevant parameters of qRT-PCR
From: Irisin deletion induces a decrease in growth and fertility in mice
Gene | Primers | Accession number | Tm(°C) | qPCR efficiency(%) |
|---|---|---|---|---|
GAPDH | F:5′-GGAGCGAGATCCCTCCAAAAT-3′ | Nm_002046.7 | 60 | 95.2 |
R:5′- GGCTGTTGTCATACTTCTCATGG-3′ | ||||
Fndc5 | F:5′ -TGGTAATCCCTGGACTGCAG-3′ | NM_153756.3 | 60 | 92.7 |
R:5′- GGGTACAAGGAGATGGAGGG-3′ | ||||
Akr1c18 | F:5′- ACCAAATTGGCAATTGAAGCT-3′ | NM_134066.3 | 60 | 89.6 |
R:5′- TGGGATCACTTCCTCACCTG-3′ | ||||
Mamld1 | F: 5′-TTCTGCAGCAGATGATGCAG-3’ | NM_005491.5 | 60 | 90.3 |
R:5′- GAGGATCTTGCCTGCTAGTG-3’ | ||||
Cyp19a1 | F:5′- ATGTGGACGTGTTGACCCTTCT-3’ | NM_000103.4 | 60 | 91.5 |
R:5′- AGGAGAGCT TGCCATGCATCAA-3’ |