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Fig. 8 | Reproductive Biology and Endocrinology

Fig. 8

From: Irisin deletion induces a decrease in growth and fertility in mice

Fig. 8

The effects of irisin on the expression of Akr1c18, Mamld1 and Cyp19a1 in vitro. KGN cells were cultured in the absence or presence of irisin (10–30 nM) for 48 h. a: Irisin increased the mRNA levels of Akr1c18, Mamld1 and Cyp19a1 (data from qRT-PCR). The mRNA levels were normalized to the GAPDH mRNA levels. Data are presented as the mean ± SD (n = 3). b, c: After exposure of KGN cells to the indicated concentrations of irisin for 72 h, the protein levels of Akr1c18, Mamld1 and Cyp19a1 were determined by Western blot analysis, and protein expression levels were quantified. d: mRNA was detected 48 h after siRNA transfection. e, f: Fndc5, Akr1c18, Mamld1 and Cyp19a1 expression at the protein level was detected 72 h after transfection (n = 3); *P < 0.05 compared to the control group

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