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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Down‐regulation of the Sp1 transcription factor by an increase of microRNA-4497 in human placenta is associated with early recurrent miscarriage

Fig. 2

Expression levels of predicted target genes in the trophoblasts after the transfection with miRNA-4497 agomir. a Levels of miRNA-4497 and mRNAs of 12 predicted target genes measured by real-time PCR after the transfection of HTR-8/SVneo cells with miRNA-4497 agomir or scrambled sequences used as a negative control (NC). Note the significant reduction in SP1, CXCR5 and BMP8A mRNA levels after overexpression of miRNA-4497. b The protein expression levels of SP1, CXCR5 and BMP8A determined by Western blotting after transfection of BeWo and HTR-8/SVneo trophoblast cell lines with miRNA-4497 agomir or negative control (NC). Note the significant decrease in the protein levels of SP1 and CXCR5 after overexpression of miRNA-4497. *, P<0.05; **, P<0.01; ***, P<0.001

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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