Fig. 4

Chitooligosaccharides (COS) administration prevents the loss of different follicle types and decreases atresia caused by cyclophosphamide (CY)/ busulfan (BUS) treatment. a Representative images of HE stained sections from control, CY/BUS and CY/BUS+COS mice, the scale bar is 200 μm. b The number of follicles at different levels was counted by tissue section. c Quantity changes of the percentage of primordial and atretic follicles in total follicles. Data are presented as mean ± SEM for three independent experiments. ** P < 0.01, and ***P < 0.001, vs. the control group, ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. the CY/BUS group