Table 1 Primer sequences for gene expression by qRT-PCR
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Accession No | Product size (bp) |
|---|---|---|---|---|
Aromatase | GCCTGTCGTGGACTTGGT | GGTAAATTCATTGGGCTTGG | NM_017085.2 | 142 |
FSHβ | AGACCAAACACCCAGAAAG | TCACTATCACACTTGCCACA | NM_001007597.2 | 140 |
LHβ | CATAGTCTCCTTTCCTGTGGC | CATTGGTTGAGTCCTGGGA | NM_012858.2 | 91 |
GnRH | GGCTTTCACATCCAAACAGAATG | TGATCCTCCTCCTTGCCCAT | NM_012767.2 | 181 |
GnRHR | TCAGGACCCACGCAAACTAC | CTGGCTCTGACACCCTGTTT | NM_031038.3 | 182 |
Kiss 1 | TGCTGCTTCTCCTCTGTGTGG | ATTAACGAGTTCCTGGGGTCC | NM_181692.1 | 110 |
Kiss 1R | CTTTCCTTCTGTGCTGCGTA | CCTGCTGGATGTAGTTGACG | NM_023992.1 | 102 |
GAPDH | GTGCCAGCCTCGTCTCATAG | CGTTGATGGCAACAATGTCCA | NM_017008.4 | 122 |