Fig. 5

Effect of hindlimb unloading on HPG axis gene expression by RT-PCR. a: hypothalamic mRNA expression levels of GnRH, Kiss and KissR in control and HU group. ***P < 0.001 and **P < 0.01 by Student’s t-test respectively. b: The mRNA expression levels of GnRHR, FSHβ and LHβ in the pituitary glands of the control and HU group. *P < 0.05 and **P < 0.01 by Student’s t-test respectively. c: Testicular mRNA expression levels of Kiss, KissR and aromatase enzyme in both control and HU group. *P < 0.05 and **P < 0.01 by Student’s t-test respectively