Fig. 4

ARHGAP19 is regulated by miR-192-5p. a MiR-192-5p expression in mouse uteri during early pregnancy. b MiR-192-5p expression in human EEC lines. c Putative binding sites of miR-192-5p in the 3’UTR region of ARHGAP19 in mice and humans. d A schematic representation of the structure of the wild-type and mutant Arhgap19-3’UTR vector. The mutant vector was generated by mutation of the putative binding sites of miR-192-5p in the 3′UTR region. e Relative luciferase activity in HEK293T cells co-transfected with miR-192-5p mimics, scrambled control, and the blank, wild-type (wt) or mutant (mut) Arhgap19 3’UTR vectors. f MiR-192-5p regulates ARHGAP19 expression in mouse uteri (left) and human EEC lines (right). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001