Fig. 2

ARHGAP19 overexpression induces the remodeling of junctional complexes. a Representative images of HEC-1-A cells transfected with ARHGAP19 or scrambled control (Scr). Scale bar, 100 µm. Arrows point to the boundaries of cell colonies. b Detection of E-cadherin expression in HEC-1-A cells after transfection of ARHGAP19. c 3D reconstruction of E-cadherin localization by IF in ARHGAP19 overexpressing cells. Scale bar, 10 µm. * represent the apex side of cell. d 3D reconstruction of Occludin localization by IF in ARHGAP19 overexpressing cells. Scale bar, 10 µm. * represent the apex side of cell. Arrows pointed to the Occludin in the cell-cell contact