Fig. 4

Effect of TPP1 siRNA knockdown on stromal cell decidualization and gene expression. Day 12 decidualization of HESC treated with either (a) estrogen only, (b) estrogen with MPA, or (c) estrogen with MPA and TPP1 siRNA transfection. HESC were treated with estrogen or estrogen with MPA (E + MPA) for 12 days before measuring TPP1 RNA and siRNA knockdown efficiency confirmed by qPCR (d). Effect of E + MPA on expression of PRL (e), IGFBP1 (f) and TPP1 (g). Expression of decidualization markers PRL (h) and IGFBP1 (i) were compared between E + MPA treated HESC that has been transfected with either TPP1 siRNA or a scrambled (scrmb) siRNA control. Scale bar = 400 μm. Analysis via paired student t-test or paired ANOVA test with significant threshold of p < 0.05. * = p < 0.05, ** = p < 0.01, *** = p < 0.001