Peptide/protein target | Name of Antibody | Manufacturer, catalog #, and/or name of individual providing the antibody | Species raised in; monoclonal or polyclonal | Dilution used |
---|---|---|---|---|
Ovine LHβ | oBetaLH | National Institute of Diabetes & Kidney Diseases (NIDDK) | Rabbit; polyclonal | 1:50 K |
Ovine GnRH-R | ovine GnRH-R | Dr. Donal Skinner (University of Wyoming) | Rabbit, polyclonal | 1:1 K |
Androgen receptor | AR(N-20) | Santa Cruz Biotechnology, SC-816 | Rabbit; polyclonal | 1:1 K |
Estrogen receptor α | ERα (Clone 1D5) | Thermo Scientific, MS-354 | Mouse; monoclonal | 1:500 |
Insulin receptor β | IRβ (Ab-6; Clone CT-3) | Thermo Scientific, MS-636 | Mouse, monoclonal | 1:200 |
PTEN | PTEN (D4.3) XP | Cell Signaling, #9188 | Rabbit, monoclonal | 1:1 K |
GAPDH | GAPDH (14C10) | Cell Signaling, #3683 | Rabbit, monoclonal | 1:1 K |
Phospho-AKT | p-AKT (Thr308) (D25E6) | Cell signaling, #13038 | Rabbit, monoclonal | 1:1 K |
AKT | AKT 9pan) (C67E7) | Cell signaling, #4691 | Rabbit, monoclonal | 1:1 K |
Phospho-mTOR | p-mTOR (S2448) | Cell signaling, #2971 | Rabbit, monoclonal | 1:1 K |
mTOR | mTOR | Cell signaling, #2972 | Rabbit, monoclonal | 1:1 K |
Phospho-ERK | p-p44/42 MAPK (T202/Y204) (D13.14.4E) | Cell signaling, #4370 | Rabbit, monoclonal | 1:1 K |
ERK | p44/42 MAPK (Erk 1/2) (137F5) | Cell signaling, #4695 | Rabbit, monoclonal | 1:1 K |