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Table 1 List of antibodies used for Western blot procedures in the present study

From: Developmental programming: gestational testosterone excess disrupts LH secretion in the female sheep fetus

Peptide/protein target

Name of Antibody

Manufacturer, catalog #, and/or name of individual providing the antibody

Species raised in; monoclonal or polyclonal

Dilution used

Ovine LHβ

oBetaLH

National Institute of Diabetes & Kidney Diseases (NIDDK)

Rabbit; polyclonal

1:50 K

Ovine GnRH-R

ovine GnRH-R

Dr. Donal Skinner (University of Wyoming)

Rabbit, polyclonal

1:1 K

Androgen receptor

AR(N-20)

Santa Cruz Biotechnology, SC-816

Rabbit; polyclonal

1:1 K

Estrogen receptor α

ERα (Clone 1D5)

Thermo Scientific, MS-354

Mouse; monoclonal

1:500

Insulin receptor β

IRβ (Ab-6; Clone CT-3)

Thermo Scientific, MS-636

Mouse, monoclonal

1:200

PTEN

PTEN (D4.3) XP

Cell Signaling, #9188

Rabbit, monoclonal

1:1 K

GAPDH

GAPDH (14C10)

Cell Signaling, #3683

Rabbit, monoclonal

1:1 K

Phospho-AKT

p-AKT (Thr308) (D25E6)

Cell signaling, #13038

Rabbit, monoclonal

1:1 K

AKT

AKT 9pan) (C67E7)

Cell signaling, #4691

Rabbit, monoclonal

1:1 K

Phospho-mTOR

p-mTOR (S2448)

Cell signaling, #2971

Rabbit, monoclonal

1:1 K

mTOR

mTOR

Cell signaling, #2972

Rabbit, monoclonal

1:1 K

Phospho-ERK

p-p44/42 MAPK (T202/Y204) (D13.14.4E)

Cell signaling, #4370

Rabbit, monoclonal

1:1 K

ERK

p44/42 MAPK (Erk 1/2) (137F5)

Cell signaling, #4695

Rabbit, monoclonal

1:1 K