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Table 1 List of antibodies used for Western blot procedures in the present study

From: Developmental programming: gestational testosterone excess disrupts LH secretion in the female sheep fetus

Peptide/protein target Name of Antibody Manufacturer, catalog #, and/or name of individual providing the antibody Species raised in; monoclonal or polyclonal Dilution used
Ovine LHβ oBetaLH National Institute of Diabetes & Kidney Diseases (NIDDK) Rabbit; polyclonal 1:50 K
Ovine GnRH-R ovine GnRH-R Dr. Donal Skinner (University of Wyoming) Rabbit, polyclonal 1:1 K
Androgen receptor AR(N-20) Santa Cruz Biotechnology, SC-816 Rabbit; polyclonal 1:1 K
Estrogen receptor α ERα (Clone 1D5) Thermo Scientific, MS-354 Mouse; monoclonal 1:500
Insulin receptor β IRβ (Ab-6; Clone CT-3) Thermo Scientific, MS-636 Mouse, monoclonal 1:200
PTEN PTEN (D4.3) XP Cell Signaling, #9188 Rabbit, monoclonal 1:1 K
GAPDH GAPDH (14C10) Cell Signaling, #3683 Rabbit, monoclonal 1:1 K
Phospho-AKT p-AKT (Thr308) (D25E6) Cell signaling, #13038 Rabbit, monoclonal 1:1 K
AKT AKT 9pan) (C67E7) Cell signaling, #4691 Rabbit, monoclonal 1:1 K
Phospho-mTOR p-mTOR (S2448) Cell signaling, #2971 Rabbit, monoclonal 1:1 K
mTOR mTOR Cell signaling, #2972 Rabbit, monoclonal 1:1 K
Phospho-ERK p-p44/42 MAPK (T202/Y204) (D13.14.4E) Cell signaling, #4370 Rabbit, monoclonal 1:1 K
ERK p44/42 MAPK (Erk 1/2) (137F5) Cell signaling, #4695 Rabbit, monoclonal 1:1 K