Fig. 5

Effects of gestational T excess and pharmacological intervention with an androgen antagonist (flutamide) or an insulin sensitizer (rosiglitazone) on insulin signaling in the pituitary from female fetuses. Representative Western blots and mean (± SEM) protein level ratio of IR-β/GAPDH (panel a), PTEN/GAPDH (panel b), p-AKT/AKT (panel c), AKT/GAPDH (panel d), p-mTOR/mTOR (panel e), mTOR/GAPDH (panel f), p-ERK/ERK (panel g), and ERK/GAPDH (panel h) in the anterior pituitary from females fetuses treated with prenatal vehicle (C, n = 10), prenatal T (T, n = 6), prenatal T and prenatal flutamide (TF, n = 6), and prenatal T and prenatal rosiglitazone (TR, n = 6). None of the group comparisons was statistically significant (n.s)