Fig. 5
Roles of CsA in H2O2-induced activation of p38, JNK and ERK1/2 in JEG-3 cells. a JEG-3 cells were pretreated with 1 μM CsA in serum-free medium for 24 h and then stimulated with 500 μM CsA for 15 min. The phosphorylation of p38, JNK and ERK1/2 was evaluated by western blot analysis. b Quantitative analysis of the phosphorylation levels of p38, JNK and ERK1/2. c The viability of the JEG-3 cells after treatment with hesperetin or anisomycin. d Apoptosis of the JEG-3 cells after treatment with hesperetin or anisomycin. The data presented are representative of three independent experiments. *p < 0.05 compared to the control; # p < 0.05 compared to the H2O2-treated group; △ p < 0.05 compared to the group treated with CsA and H2O2