|
Inhibitor
|
Concentration
|
No. oocytes
|
Repeats
|
PA
|
PN
|
2-Cell
|
|---|
|
Ctrl
|
240
|
5
|
98.33 ± 1.24%
|
93.25 ± 5.32%
|
82.90 ± 7.53%
|
|
Tha
|
0.5 μM
|
240
|
5
|
84.2 ± 5.72%
|
65.7 ± 9.56%*
|
67.6 ± 7.59%
|
|
1 μM
|
240
|
5
|
66.2 ± 9.24%
|
42.1 ± 8.56%*
|
26.1 ± 12.45%*
|
|
10 μM
|
240
|
5
|
0
|
X
|
X
|
|
NS-8593
|
0.1 μM
|
240
|
5
|
83.3 ± 6.28%
|
75.2 ± 9.57%
|
86.7 ± 7.50%
|
|
1 μM
|
240
|
5
|
37.5 ± 12.53%*
|
52.9 ± 7.84%*
|
57.7 ± 11.56%*
|
|
5 μM
|
240
|
5
|
0
|
X
|
X
|
|
Mib
|
0.5 μM
|
240
|
5
|
77.3 ± 5.74%
|
55.6 ± 10.60%
|
67.5 ± 8.82%
|
|
5 μM
|
240
|
5
|
36.7 ± 5.68%*
|
42.5 ± 5.93%*
|
66.4 ± 10.34%
|
|
10 μM
|
240
|
5
|
3.6 ± 2.40%*
|
0
|
X
|
|
GSK-7975
|
10 μM
|
240
|
5
|
95.4 ± 3.21%
|
84.5 ± 6.34%
|
82.7 ± 7.26%
|
|
100 μM
|
240
|
5
|
83.8 ± 5.21%
|
82.6 ± 5.83%
|
58.4 ± 11.57%
|
|
1 mM
|
240
|
5
|
63.5 ± 7.95%*
|
0
|
X
|
- Note:Tha indicates SERCAs inhibitor Thapsigargin. NS-8593 is a TRPM7 specific inhibitor. Mib indicates T-type Ca2+ channels inhibitor Mibefradil. GSK-7975A is an Orai1 specific inhibitor. PA indicates survival embryos of all activated oocytes after activation. PN indicates pronuclear embryos from surviving oocytes. 2-Cell indicates cleaved embryos in pronuclear fertilized eggs. “X” indicates none available data. The significance of differences between Inhibitors and Ctrl group were analyzed by the Chi-square test and p < 0.05 (*) was considered statistically significant
