Fig. 4
Effect of T-type Ca2+ channel inhibitor Mibefradil on oocyte activation. a [Ca2+]i oscillations of Mibefradil-inhibited oocytes. b Mitochondrial membrane potential dynamic changes of 5 μM Mibefradil-inhibited oocytes. c Mitochondrial membrane potential fluorescence intensity of 5 μM Mibefradil-inhibited oocytes. The green and red curves represent low and high membrane potential, respectively. The black curve shows the ratio of high membrane potential to low membrane potential indicating relative mitochondrial membrane potential. d Living cell observation of 5 μM Mibefradil inhibition of oocyte spindle formation e Living cell observation of 5 μM Mibefradil inhibition of oocyte subcortical F-actin distribution