Fig. 1
From: Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis
Experimental design and the workflow in this study. Two sets of biological replicate samples were analyzed by iTRAQ, using the hpRP-nanoLC-MS/MS workflow for examining proteome changes between human sperms of 2 weeks before the first treatment (control) and 6 weeks after the first local heat stress of the scrotum (Heat treatment 6w). Proteins were identified using Protein Pilot Software