Fig. 3

FOXP3 protein and mRNA expression differs between URSA patients and normal control subjects. a Comparative analysis of the mean fluorescence intensity of FOXP3 expression in peripheral CD4⁺CD25^highCD127^low/− Tregs in normal controls (n = 12) and URSA patients (n = 12) (**P < 0.01). b Comparative analysis of FOXP3 mRNA expression in peripheral CD4⁺CD25^highCD127^low/− Tregs from normal control subjects (n = 12) and URSA patients (n = 12) (**P < 0.01) by quantitative reverse-transcription PCR. Relative expression was calculated using the 2^−ΔΔCT method