Fig. 5

DANCR functions as a competing endogenous RNA to sponge the functions of miR-33a-5p in pancreatic β cells. a The predicted binding sites of miR-33a-5p and DANCR were analyzed by starBase v2.0. b The luciferase activity was analyzed in HEK293T cells co-transfected with wild-type DANCR (DANCR-WT) or mutated DANCR (DANCR-Mut.) and miR-33a-5p mimic or mimic NC. n = 3. c The abundance of miR-33a-5p was analyzed in INS-1 cells transfected with vector, DANCR, siNC or siDANCR. n = 3. d CCK-8 assay was measured in INS-1 cells co-transfected with DANCR, empty vector, miR-33a-5p mimic or mimic NC. n = 6. e DANCR, empty vector, miR-33a-5p mimic or mimic NC were transfected into INS-1 cells for 48 h. Insulin content was determined by ELISA assay. n = 6. ^**p < 0.01, ^##p < 0.01, n.s. means no significance