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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: MicroRNA-33a-5p sponges to inhibit pancreatic β-cell function in gestational diabetes mellitus LncRNA DANCR

Fig. 4

miR-33a-5p targets ABCA1 and inhibits its expression in pancreatic β cells. a The binding sites of miR-33a-5p in ABCA1 3’UTR was predicted online (http://www.microrna.org). b MiR-33a-5p mimic and mimic NC, along with wild-type (WT) or mutant (Mut.) ABCA1 3’UTR were co-transfected into HEK293T cells for 48 h, followed by luciferase assay. n = 3. INS-1 cells were transfected with mimic NC or miR-33a-5p mimic for 48 h, AMCA1 expressions were examined by qRT-PCR (c) (n = 3) and immunoblotting (d)n = 3. e The expression levels of ABCA1 in peripheral blood samples from GDM pregnancies (n = 12) and healthy pregnancies (n = 12) were validated by qRT-PCR. **p < 0.01

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