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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

Fig. 2

Exposure to CSC-triggered cell death in GC-2spd cells. a GC-2spd cells were treated with 0–800 μg/mL of CSC for 24 h. Cell viability was measured by CCK-8 assay (n = 3). b The viability of GC-2spd cells were detected after treatment with 400 μg/mL of CSC, we also detected the cellular viability after the treatment with Fer-1 and Z-VAD-fmk to eliminate their cytotoxicity. And the cellular viability following pre-incubation with 400 μg/mL of CSC plus 10 μmol/L of Fer-1, Z-VAD-fmk and Fer-1 combined with Z-VAD-fmk for 24 h was detected respectively. *P < 0.05,**P < 0.01 represents the statistical difference compared to the respective group (n = 3)

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