Combined use of Diane-35 and metformin improves the ovulation in the PCOS rat model possibly via regulating glycolysis pathway

Table 1 Primer sequences of the qRT-PCR analysis

Gene	Sequence (5′-3′)	Annealing Temperature	Product (bp)	GeneBank No
PCNA	F: GCTCCATCCTGAAGAAGGT	55 °C	121	NM-022381.3
	R: TGCACTAAGGAGACGTGAGA
Bax	F: GAGACACCTGAGCTGACCTT	55 °C	104	NM-017059.2
	R: TCCATGTTGTTGTCCAGTTC
Bcl-2	F: AGTACCTGAACCGGCATCT	55 °C	120	NM-016993.1
	R: CCGGTTACTATTCCTGGAGA
Caspase-3	F: CCGGTTACTATTCCTGGAGA	55 °C	117	NM-017008.4
	R: TAACACGAGTGAGGATGTGC
MCT4	F: CCTTCCTTCTCACCATCCT	55 °C	136	BC168146.1
MCT4	R: TCAGTGAAGCCATTGAAGAA	55 °C	136	BC168146.1
MCT2	F: GGATTGGGATTTGGAAGTAT	55 °C	119	NM-003676.8
MCT2	R: AGAACTGGACAACACTCCAC	55 °C	119	NM-003676.8
GLUT1	F: TGGCTCCTCATGTCAGAGA	55 °C	125	AJ245935.1
GLUT1	R: AGGATCTCCATGATGCTGTT	55 °C	125	AJ245935.1
HK	F: AGAGGCTACGGACAGAGATG	55 °C	121	NM-012734.1
HK	R: AGGAAGTCACCGTGTTCAGT	55 °C	121	NM-012734.1
PFK	F: GGCGTGTGTTCATTGTAGAG	55 °C	125	NM-017008.4
PFK	R: CTTCAAGTCGTGGATGTTGA	55 °C	125	NM-017008.4
PKM	F: ACATCCTGTGGCTGGACTAT	55 °C	130	NM-053297.2
PKM	R: TCCACTTCTGTCACCAGGTA	55 °C	130	NM-053297.2
LDH	F: GGTTGACAGTGCATACGAAT	55 °C	106	NM-017025.1
LDH	R: CCGCCTAAGGTTCTTCATTA	55 °C	106	NM-017025.1
GAPDH	F: CCTCAAGATTGTCAGCAATG	55 °C	134	NM-017008.4
GAPDH	R: CAGTCTTCTGAGTGGCAGTG	55 °C	134	NM-017008.4

ISSN: 1477-7827