Table 1 Primers used for Real-time PCR
From: Prostaglandin E2 affects in vitro maturation of bovine oocytes
Gene Symbol | Gene Name | Primer sequence (5′-3′) | Fragment size, bp | Gen Bank accession no. |
|---|---|---|---|---|
PTGS2 | prostaglandin endoperoxide synthase 2 | TGGGTGTGAAAGGGAGGAAA AAGTGCTGGGCAAAGAATGC | 127 | NM_174445.2 |
PTGES | microsomal prostaglandin E synthase 1 | TGCTGGTCATCAAAATGTACG GCAGTTTCCCCAGGTATGC | 300 | NM_174443.2 |
PTGES2 | microsomal prostaglandin E synthase 2 | CCTCCTACAGAAAGGTGCC GTGATGATGTCTGCCAGGG | 133 | NM_001166554.1 |
PTGES3 | cytosolic prostaglandin E synthase | TGCAAAGTGGTACGATCGG TAACCTTGGCCATGACTGG | 253 | NM 001007806 |
PTGER1 | prostaglandin E2 receptor 1 | GAGTCCCTTGCTGGTGGTGGT CTGGTTCCACGAGGCGAGGC | 103 | NM_001192148.1 |
PTGER2 | prostaglandin E2 receptor 2 | GCTCCTTGCCTTTCACGATTTT CTCAGGATGGCAAAGACCCAA | 130 | NM_174588.2 |
PTGER3 | prostaglandin E2 receptor 3 | CATACTGGGGCTCTCGGCG TCATTATCAGCAACGGCGACCA | 200 | NM_181032.1 |
PTGER4 | prostaglandin E2 receptor 4 | CATCCCGCTTGTGGTGCGAG AGGGTTTTTGCCGATGACTGG | 76 | NM_174589.2 |
CTSB | cathepsin B | GGCTCACCCTCTCCAGTCCT TCACAACCGCCTTGTCTGAA | 136 | NM 174031.2 |
CTSK | cathepsin K | GAACCACTTGGGGGACATGA GGGAACGAGAAGCGGGTACT | 77 | NM 001034435.1 |
CTSS | cathepsin S | CCGCCGTCAGCATTCTTAGT CATGTGCCATTGCAGAGGAG | 99 | NM 001033615.1 |
CTSZ | cathepsin Z | GGGGAGGGAGAAGATGATGG CCACGGAGACGATGTGGTTT | 146 | NM 001077835.1 |
GLUT1 | glucose transporter 1 | GATCCACAGAGCGCAGCC TGTCAGCTTCTTGCTGGTGG | 90 | NM_174602.2 |
GLUT4 | glucose transporter 4 | ATTGTGGCCATCTTTGGCTTCGTG AACCCATGCCGATGATGAAGTTGC | 160 | NM_174604.1 |
GFPT1 | glutamine-fructose-6-phosphate transaminase 1 | AAACACAGTCGGCAGTTCCA TGGCTACACCAATCTCAGGC | 80 | NM_001109961.1 |
GFPT2 | glutamine-fructose-6-phosphate transaminase 2 | GAGATGTGCGGAATCTTTGCC ACCTGCTGAGTCATAGCCTCT | 120 | NM_001076883.1 |
PFKP | phosphofructokinase | TCAGAGAACCGTGCCTGGAAGAAA TGACCACAAGCTCCTTGATCTGCT | 112 | NM_001193220.1 |
LDHA | lactate dehydrogenase A | TCTGGATTCAGCTCGCTTCCGTTA TTCTTCAGGGAGACACCAGCAACA | 147 | NM_174099.2 |
CAT | catalase | CTGGGACCCAACTATCTCCA GATGCTCGGGAGCACTAAAG | 112 | NM_001035386.2 |
GPX4 | glutathione peroxidase 4 | GTGCTCGCTCCATGCACGA CCTGGCTCCTGCCTCCCA | 120 | NM_174770.4 |
FAS | Fas cell surface death receptor | AAAAACTGGGGCTGCCCTTA CTTTGTGGGGGATGGAACAA | 148 | NM_174662.2 |
FASLG | Fas ligand | ACTACCGCCACCACCTCTGA GGCCACCAGAACCATGAAAA | 85 | NM_001098859.1 |
TNFα | tumor necrosis factor alpha | CCGCATTGCAGTCTCCTACC TGGGTTCATACCAGGGCTTG | 110 | NM_173966.2 |
TNFR1 | tumor necrosis factor receptor 1 | CCACTGGTGCTTCCAGCTCT TTTCCTTGGGGACAGGGACT | 110 | NM_174674.2 |
TNFR2 | tumor necrosis factor receptor 2 | CCCCAGGACTCTGGCTCTTT CCAAGACAGGACCCATCAGG | 113 | NM_001040490.1 |
BAX | BCL2 associated X, apoptosis regulator | GTGCCCGAGTTGATCAGGAC CCATGTGGGTGTCCCAAAGT | 126 | NM_173894.1 |
BCL2 | BCL2 apoptosis regulator | GAGTTCGGAGGGGTCATGTG GCCTTCAGAGACAGCCAGGA | 203 | NM_001166486.1 |
CASP8 | caspase 8 | CTGAGAGAAGAGGCCCGTGA CCCGGCTTAGGAACTTGAGG | 173 | NM_001045970.2 |
CASP3 | caspase 3 | TGGTGCTGAGGATGACATGG GAGCCTGTGAGCGTGCTTTT | 163 | NM_001077840.1 |
GAPDH | glyceraldehyde-3-phosphate dehydrogenase | CACCCTCAAGATTGTCAGCA GGTCATAAGTCCCTCCACGA | 103 | NM_001034034.2 |