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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Prostaglandin E2 affects in vitro maturation of bovine oocytes

Fig. 3

The effect of PGE2 (10 μM) treatment of oocyte maturation medium on (a) glucose uptake and (b) lactate production by COCs, and on mRNA abundance of genes involved in glucose metabolism: cGLUT1, dGLUT4, eGFPT1, fGFPT2, gPFKP, and hLDHA in cumulus cells. Glucose uptake and lactate production are expressed as mean ± SEM of six independent experiments and presented as mg/dl per COC and mmol/L per COC, respectively. The values of mRNA expression are presented as arbitrary units and expressed as mean ± SEM of eight independent repeats (all cumulus cells separated from 10 respective oocytes in each replicate). Different letters indicate significant differences (P < 0.05), as determined by Student’s t-test

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