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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Molecular analysis of lipid uptake- and necroptosis-associated factor expression in vitrified-warmed mouse oocytes

Fig. 4

Localization of pMLKL in vitrified-warmed oocytes from young and aged mice. a Specificity of anti-pMLKL antibody was confirmed in L929 cells treated with necroptosis-inducing reagents. -, no treatment; TSZ, a mixture of TNFα, LCL161, Z-VAD-FMK. Scale bar represents 50 μm. b Immunofluorescence staining of pMLKL in oocytes from young and aged mice before (fresh) and after vitrification-warming (V/W). Oocytes were fixed, permeabilized, and treated with anti-pMLKL antibody at 1:100 (green). DNA was counterstained with TOPRO-3-iodide (1:250). Experiments were repeated three times, using more than 14 oocytes per group. White scale bar represents 20 μm. c A representative image showing co-localization of pMLKL and pericentrin. The white boxed area is enlarged four times. The primary antibodies used are, anti-pMLKL (1:100, green) and anti-pericentrin (1:500, red). The arrow indicates the spindle pole and the arrowhead indicates kinetochore. White scale bar represents 10 μm

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