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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: MiR-423-5p may regulate ovarian response to ovulation induction via CSF1

Fig. 1

a Transfection with the lentivirus or inhibitor caused hsa-miR-423-5p expression to be increased or decreased in KGN cells, respectively. The black bars were controls, and the grey bar was hsa-miR-423 transfection group or hsa-miR-423-5p inhibitor transfection group. (*p < 0.05). b The predicted sequences to which hsa-miR-423-5p binds in the 3′-UTR of CSF1 are shown in yellow shadow. c Hsa-miR-423-5p negatively regulates the expression of CSF1. Hsa-miR-423-5p overexpression led to reduced CSF1 expression, whereas the use of inhibitors led to increased CSF1 expression, in KGN cells. d Overexpression of hsa-miR-423-5p reduced the expression of CSF1 protein, and decreased expression of hsa-miR-423-5p increased the expression of CSF1 protein. The bar graphs indicate the mean ± SEM. The black bars were controls, and the grey bar were hsa-miR-423 transfection groups or hsa-miR-423-5p inhibitor transfection groups (*p < 0.05,** p < 0.01)

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