Fig. 3

IR-β in a ITf, b STf and c SPZ in db/db, ob/ob and wt mice. The values are the means ± SEM; n = 3 animals per age group. a In ITf, the drop in 98-110 kDa band levels was significant in (cP < 0.000002) db/db and (dP < 0.000003) ob/ob mice compared to the wt counterparts. 75-kDa band levels fell significantly in db/db (mP < 0.0000001) but significantly increased (##P < 0.002) in ob/ob mice compared to the wt counterparts. Compared to the wt counterparts, 50 kDa band (mP < 0.0000001) and Total IR-β protein levels (mP < 0.0000001) significantly decreased in db/db mice; as well, in ob/ob mice, 50 kDa band (nP < 0.0000005) and Total IR-β protein levels (iP < 0.00003) significantly dropped. b In STf, 98-110 kDa band levels significant fell in db/db (‡‡P < 0.003) and ob/ob (††P < 0.001) compared to the wt counterparts. Contrarily, 75-kDa band levels significantly increased (bP < 0.00005) in db/db and ob/ob mice (##P < 0.002) compared to the wt counterparts. The 66 kDa band remained relatively unchanged in db/db but significantly augmented in ob/ob (*P < 0.05) mice in comparison to the wt counterparts. Fifty kDa significantly dropped in db/db (††P < 0.001) and ob/ob (††P < 0.001) compared to wt mice. Total IR-β protein levels significantly upsurged in (**P < 0.005) db/db but dropped in (††P < 0.001) ob/ob compared to the wt counterparts. c In SPZ, 98-110 kDa band significant fell in (*** P < 0.0005) db/db and (** P < 0.005) ob/ob compared to the wt counterparts. The decrease in 66 kDa band was not significant compared to the wt but the decrease in the 50 kDa band was significant (*** P < 0.0005) in db/db and ob/ob mice. Total IR-β protein levels significantly decreased in db/db (†P < 0.01) and ob/ob (#P < 0.02) compared to the wt counterparts. IR-α in d ITf, e STf and e SPZ. The values are the means ± SEM; n = 3 animals per age group. d In ITf, the decrease in 135 kDa band was significant in (**P < 0.005) db/db and (*P < 0.05) ob/ob and the drop in 90 kDa band was significant only in (##P < 0.002) db/db compared to the wt counterparts. The fall in 75 kDa band was significant in (†††P < 0.0001) db/db and (#P < 0.02) ob/ob. Fifty kDa band significantly increased in (†P < 0.01) db/db while 36 kDa band significantly decreased in both (*P < 0.05) db/db and ob/ob compared to wt counterparts. However, Total IR-α protein levels were similar in all groups. e In STf, 135 kDa band levels were not significantly different in db/db and ob/ob compared to the wt counterparts however, the increase in 90 kDa band was significant in (*P < 0.05) db/db and (†P < 0.01) db/db. The fall in 75 kDa was significant in (*P < 0.05) db/db compared to the wt counterparts. The decrease in 50 kDa bands levels was not significant whereas 36 kDa bands significantly dropped in (‡P < 0.03) db/db and (#P < 0.02) ob/ob compared to the wt counterparts. The decrease in Total IR-α protein levels were not significant in comparison to wt counterparts. f In SPZ, the decrease in 90 kDa band was significant only in (***P < 0.0005) db/db and the decrease in 75 kDa was significant in (†P < 0.01) db/db compared to the wt counterparts. The 50 kDa band significantly decreased (††P < 0.001) in db/db and ob/ob as well, 36 kDa significantly decreased in (‡‡‡P < 0.0003) db/db and (†P < 0.01) ob/ob compared to wt counterparts. Total IR-α levels were significantly lowered in (***P < 0.0005) db/db and (†P < 0.01) ob/ob in comparison to the wt