Fig. 2

a and b show the glucose concentration in a the serum and b the interstitial tissue- (ITf) and seminiferous tubule-enriched fraction (STf) and in epididymal spermatozoa (SPZ) from db/db, ob/ob and wt mice. The values are the means ± SEM; n = 3 animals per age group. a The increase in circulating glucose concentration was very significant in (†††P < 0.0001) db/db and (‡‡‡P < 0.0003) ob/ob compared to the wt counterparts. b In db/db, glucose concentration significantly (‡P < 0.03) augmented in ITf but instead significantly dropped in (#P < 0.02) STf and (**P < 0.005) SPZ compared to the wt counterparts. The decrease in glucose was significant (*P < 0.05) in SPZ in ob/ob compared to the wt counterparts. c-e Insulin T and Insulin 2 in c serum, d ITf and e STf in db/db, ob/ob and wt. The values are the means ± SEM; n = 3 animals per age group. c Insulin T very significantly increased in both (bP < 0.00005) db/db and (bP < 0.00005) ob/ob sera compared to the wt counterparts. As well, Insulin 2 also very significantly escalated in (aP < 0.00001) db/db and (bP < 0.00005) ob/ob sera. d In ITf, Insulin T significantly augmented in (*P < 0.05) db/db and (#P < 0.02) ob/ob compared to the wt counterparts; similarly, Insulin2 significantly augmented in (#P < 0.02) db/db and (##P < 0.002) ob/ob. e As well, Insulin T significantly increased in STf in (*P < 0.05) db/db and (IP < 0.03) ob/ob compared to the wt