Fig. 6

Effect of flutamide (F) and EDS on Hey1, Hes1, and Hes5 expression in rat testis. (A - C) Relative expression of Hey1, Hes1, and Hes5 mRNAs was determined using real-time RT-PCR analysis. The histograms are the quantitative representation of data of three independent analyses, each in triplicate (n = 6 each group). The expression values of the individual genes were normalized to the mean expression of the reference genes (Rn18s, B2m and Actb) as an internal control. Relative quantification (RQ) is expressed as mean ± SD. Significant differences from control values are denoted as **p < 0.01 and ***p < 0.001. (A’- C′) Relative protein expression of HEY1, HES1 and HES5 was determined using western blot. The histograms are the quantitative representation after densitometry of data (mean ± SD) of three independent analyses (n = 6 each group). The relative level of studied protein was normalized against its corresponding actin data point. The protein levels within the control group were arbitrarily set at 1. Significant differences from control values are denoted as *p < 0.05, **p < 0.01, and ***p < 0.001