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Table 4 Spearman’s rank-order correlation between bacteria presented in raw semen and washed sperm (*) detected by pyrosequencing (454), qPCR and clinical data

From: The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure

BacteriaClinical dataRP
Presence of Bacteroidia (454, class)Neutrophils0.6730.0028
Presence of Erysipelotrichia (454, class) 0.5230.0309
Erysipelotrichia (454, OTU numbers) 0.6360.006
Counts of Staphylococcus spp. (qPCR) 0.4480.002
Total OTU numbers (454, phylum)Sperm motility−0.4720.0551
Bacteroidetes (454, phylum, OTU numbers) −0.5180.0328
Bacteroidia (454, class, OTU numbers) −0.4980.0411
Proteobacteria (454, phylum, OTU numbers) −0.6610.0036
Alphaproteobacteria (454, class, OTU numbers) −0.4710.0551
Sphingobacteria (454, class, OTU numbers) −0.6350.0006
Alphaproteobacteria (454, OTU numbers)*Embryo quality (Grade 1, 2, 3)0.4130.0260