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Table 3 Correlation matrix between % of capacitated boar sperm (chilled 17 °C/diluted) after 240 min of incubation detected by individual methods and number of cells after ZP-induced acrosome reaction detected by PSA; n = 20

From: Fluorescent analysis of boar sperm capacitation process in vitro

 

PSA FMi

PSA FCj

ACR.2 FMa

0.92

0.69

ACR.2 FCb

0.77

0.84

CTC FMc

0.93

0.72

CTC FCd

0.19

0.21

Phall FMe

0.68

0.35

Phall FCf

0.54

0.62

anti-pY FMg

0.5

0.60

anti-pY FCh

0.64

0.71

  1. Correlation coefficients (r) between detections of capacitation status by individual detection methods and detection of cells after ZP-induced acrosome reaction detected by appropriate methods
  2. aFluorescent microscopy (FM) with anti-acrosin (ACR.2) antibody
  3. bFlow cytometry (FC) with ACR.2 antibody
  4. cFluorescent microscopy with chlortetracycline (CTC FM)
  5. dFlow cytometry with chlortetracycline (CTC FC)
  6. eFluorescent microscopy with fluorescein isothiocyanate-conjugated phalloidin (Phall FM)
  7. fFlow cytometry with fluorescein isothiocyanate-conjugated phalloidin (Phall FC)
  8. g Fluorescent microscopy with anti-phosphotyrosine antibody (anti-pY FM)
  9. hFlow cytometry with anti-phosphotyrosine antibody (anti-pY FC)
  10. i Fluorescent microscopy with Pisum sativum (PSA FM)
  11. jFlow cytometry with Pisum sativum (PSA FC)
  12. Significant correlation coefficient in bold (p ≤ 0.05)