Fig. 2
From: Fluorescent analysis of boar sperm capacitation process in vitro
Percentage of non-capacitated, capacitated and acrosome-reacted sperm analyzed by FC after different incubation time in capacitation medium (0, 120 and 240 min) and ZP-induced AR. Individual bars denote the percentage of non-capacitated, capacitated and AR cells as detected by individual methods among pre-defined sequential times of the capacitation and after AR. Samples from 20 individual boars were analyzed in this assay. No significant difference among individual methods at 0 min and after AR. Significant difference between CTC/ACR.2 vs anti-pY/FITC-phall at 120 and 240 min (p ≤ 0.05). The percentage of capacitated sperm differed (p ≤ 0.05) among end points (0, 120, 240, and AR) for the same evaluation method. Error bars indicate SEM