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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Fluorescent analysis of boar sperm capacitation process in vitro

Fig. 1

Fluorescent microscopy pictures of sperm stained with CTC, ACR.2, anti-pY and FITC-phall. Acrosomal and sperm head fluorescent patterns prominent in distinct stages of capacitation process. a1 – a3 sperm treated by CTC: a1 Non-capacitated, acrosome-intact sperm - bright fluorescence over the entire sperm head and positive mid-piece of the tail; a2 Capacitated, acrosome-intact sperm - prominent fluorescent positive equatorial segment and mid-piece, fluorescence-free (dark) band in the post-acrosomal region; a3 Acrosome-reacted sperm - low fluorescent signal throughout the sperm head, with a remaining positive signal in the equatorial segment and mid-piece. B1 – B3 representative pictures of three specific ACR.2 acrosomal fluorescent patterns: b1 Non-capacitated, acrosome-intact sperm - moderate uniform fluorescence in the acrosomal area; b2 Capacitated, acrosome-intact sperm - intensive fluorescence of the acrosome; b3 Acrosome-reacted sperm - low or no fluorescent signal in the sperm head. Anti-pY: C1 – C3 pictures of three specific pY staining patterns: c1 Non-capacitated sperm – moderate signal in the acrosomal area, visible triangular segment; c2 Intensive fluorescence of the sperm head, triangular segment and tail – capacitated, acrosome-intact sperm; c3 Very low/no signal in the acrosomal area, visible triangular segment – acrosome reacted sperm. D1 – D3 representative pictures of three specific FITC-phall staining: d1 Non-capacitated sperm – moderate fluorescence in the acrosomal and sperm head/tail area; d2 Intensive fluorescence of the acrosome and the tail – capacitated, acrosome-intact sperm; d3 Low intensity in the acrosomal and apical sperm head area – sperm after AR. b1 – b3, c1 – c3 nuclei stained with a Blue DAPI dye

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