Steps | Key findings |
---|---|
Step 1 | Increased trend in publications on SDF over the past 20 years |
Step 2 | Increased number of articles reporting the association between SDF and male infertility |
Step 3 | SDF research was mainly focused on lifestyle, varicocele and asthenozoospermia while less investigated in association with testicular cancer, globozoospermia and hypogonadism |
Step 4 | Majority of the mechanistic studies were based on oxidative stress and the number of publications on mechanistic studies of SDF was twice as much as prognostic/diagnostic studies |
Step 5 | TUNEL assay was the most widely used technique to assess SDF |
Step 6 | Significant increase in publication trends of SDF-based ART studies |