Fig. 4

The percentage of pachytene oocytes peaks at PND1 as determined by SYCP3 labeling. a-c Representative confocal microscope sections labeled for SYCP3 (green) and nuclear marker propidium iodide (red) at a 13.5 dpc showing unlabeled mitotic nuclei, b 15.5 dpc showing pre-pachytene meiotic nuclei and c PND1 showing pachytene meiotic nuclei. Scale bar = 10 μm. d Percent of oocytes in the pachytene stage of development in ovaries from 13.5 dpc through PND5 determined by whole mount SYCP3 expression (+/−SEM). Different letters indicate a significant difference between groups (P < 0.05; n = 8 ovaries per developmental stage) as determined by a one-way ANOVA followed by Newman-Keuls multiple comparisons test