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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Molecular analysis of the effects of steroid hormones on mouse meiotic prophase I progression

Fig. 2

MSY2 expression increases over time in developing ovaries and is asymmetric. a Relative mRNA levels (+/−SEM) for Msy2 as determined by qPCR in perinatal mouse ovaries from 13.5 dpc to PND5. The levels of Msy2 mRNA are normalized to the levels of mRNA for a housekeeping gene, β-actin in the same sample. The normalized values are expressed relative to the mRNA levels at 13.5 dpc with 13.5 dpc set at 1. Asterisks indicate a significant difference (P < 0.0005) as determined by one-way ANOVA with Bonferroni-Dunn’s post hoc test (n = 3). b Percent of oocytes with strong MSY2 protein labeling in ovaries (+/−SEM) from 15.5 dpc through PND5. Different letters indicate a significant difference between groups (P < 0.05; n = 8 ovaries per developmental stage) as determined by a one-way ANOVA followed by Newman-Keuls multiple comparisons test. c-e Asymmetric expression of MSY2 protein in wildtype ovaries. Image shown is a confocal microscope section from a PND1 wildtype ovary. c MSY2 expression labeled in green. d Nuclei of all cells labeled with propidium iodide in red. e Overlay of both MSY2 (green) and propidium iodide (red) channels. Asterisk indicates an oocyte strongly expressing MSY2. Square indicates an oocyte with weak MSY2 expression. The arrow and arrowhead are showing an example of asymmetric expression of MSY2 within the same cyst. The arrow indicates an oocyte with weak MSY2 expression within the cyst and the arrowhead indicates an oocyte with strong MSY2 expression within that same cyst. Scale bar = 20 μm

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