Fig. 3

The effect of cortisol on the activity of the Wnt/β-catenin pathway in bovine endometrial epithelial cells. (a) Cells were treated with cortisol (15 ng/mL) for 0, 15, 30, 45 and 60 min. (b) Cells were treated with cortisol (5, 15 and 30 ng/mL) for 30 min. The β-catenin, c-Myc and cyclinD1 levels were determined by Western blotting analysis. β-actin was used as the internal control. (c) Cells were treated with cortisol (15 ng/mL) for 30 min. The β-catenin levels were evaluated by confocal microscopy. Three uteri (different sets of culture cells) were used for analysis. The data are presented as the means ± SEM. * p < 0.05, ** p < 0.01 vs the control group