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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice

Fig. 1

Ovol2 is expressed in spermatogenic cells in mice. a RT-qPCR analyses of Ovol2 mRNA levels in nine organs of adult mice. Data are presented as mean ± SEM, n = 5. b Western blot analyses of OVOL2 protein levels in nine organs of adult mice. GAPDH served as a loading control. c Quantification analyses of OVOL2 protein levels in nine organs. Data are presented as mean ± SEM, n = 3. d RT-qPCR analyses of Ovol2 mRNA levels in developing testes. Testes at postnatal Day 0 (P0), P7, P14, P21, P28, P35, and P56 were analyzed. Data are presented as mean ± SEM, n = 5. e Western blot analyses of OVOL2 protein levels in developing testes. Testes at postnatal Day 0 (P0), P7, P14, P21, P28, P35, P42 and P56 were analyzed. GAPDH served as a loading control. f Quantification analyses of OVOL2 protein levels in developing testes. Data are presented as mean ± SEM, n = 3. g Immunofluorescence showing the localization of OVOL2 in P21 and adult testis sections. Note: Spg, spermatogonia; Spc, spermatocytes; Rds, round spermatids; eLg, elongated spermatids; Ser, sertoli cells; Ley, leydig cells. Scale bar = 50 μm

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