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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: MicroRNA-451 is downregulated in the follicular fluid of women with endometriosis and influences mouse and human embryonic potential

Fig. 5

Morphology and statistical results of the 2 pronuclear (2PN), 8–10-cell, and blastocyst stages of human oocytes after injection with either miR-451 inhibitor or negative control (NC) inhibitor and oocytes of the untreated control group. Morphology of the 2PN, 8–10-cell and blastocyst stages of the miR-451 inhibitor-injected (n = 22), NC inhibitor-injected (n = 20), and untreated (n = 19) oocytes. a The 8–10-cell stage of abnormal embryos from oocytes microinjected with miR-451 inhibitor; b The 8–10-cell stage of normal embryos from oocytes microinjected with NC inhibitor; c The blastocyst stage of abnormal embryos from oocytes microinjected with miR-451 inhibitor; d The blastocyst stage of normal embryos from oocytes microinjected with NC inhibitor. Scale bar = 50 μm. The proportions of oocytes in the untreated, NC inhibitor, and miR-451 inhibitor groups that developed into the 2PN stage (e) were 72.64% ± 5.62, 61.25% ± 5.71, and 28.96% ± 3.29%, respectively; into the 8–10-cell stage (f) were 53.42% ± 4.78, 49.15% ± 4.28, and 19.81% ± 3.73%, respectively; and into the blastocyst stage (g) were 37.54% ± 3.72, 31.16% ± 4.89, and 9.23% ± 2.32%, respectively. All experiments were repeated at least thrice. Unpaired t-test; *P < 0.05; **P < 0.01; ***P < 0.001. NC, negative control

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