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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Reduced expression of PD-L1 in autoimmune thyroiditis attenuate trophoblast invasion through ERK/MMP pathway

Fig. 4

Effect of PD-L1 knockdown on cell invasion in HTR-8/SVneo cells. Parental or HTR-8/SVneo cells transfected with negative control siRNA (NC) or siRNAs (siRNA1 siRNA2) targeting PD-L1 for 48 h were seeded in modified transwell chamber with Matrigel-coated membrane, and after another 24 h, invasive cells that moved through the Matrigel membrane were stained and counted under a microscope (× 100). a Showed the nuclei of the invasive cells. b Showed statistical analysis of the number of invasive cells. Data was shown as means ± SD from five fields. **P < 0.01 versus the NC group

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